Rothamsted Research

where knowledge grows


Timothy Mauchline

Research Scientist
Sustainable Agriculture Sciences Harpenden
+44 (0) 1582 763 133 ext: +44 1582 938412
Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ
Past Projects
Project Leader
  • *BBSRC Embrapa -U/standing whea
  • The diversity, biology and dynamics of microbial agents that regulate nematode populations in the rhizosphere
  • LAT Studentship: Analysis of Escherichia Coli O157 colonisation of the plant environment
  • BBSRC CASE Studentship: Characterisation and bio-prospecting of endophytic bacterial microbes in wheat
  • Lawes Trust Studentship: Rhizobia diversity: the effect of farm management, soil type and environment
  • Optimisation of nutrients in soil-plant systems: How can we control nitrogen cycling in soil?
  • Optimisation of nutrients in soil-plant systems: Determining how phosphorus availability is regulated in soils
  • The importance of the wheat root microbiome for plant health and crop yields

Nguyen, D.D. Melnik, A.V., Koyama, N., Lu, X., Schorn, M., Fang, J., Aguinaldo, K., Lincecum, T.L. Jr, Ghequire, M.G., Carrion, V.J., Cheng T.L., Duggan, B.M., Malone, J.G., Mauchline, T.H., Sanchez, L.M., Kilpatrick, A.M., Raaijmakers, J.M., Mot, R., Moore, B.S., Medema, M.H., Dorrestein, P.C. (2016). Indexing the Pseudomonas specialized metabolome enabled the discovery of poaeamide B and the bananamides. Nat Microbiol. 2016 Oct 31;2:16197. doi: 10.1038/nmicrobiol.2016.197.




le Cocq, K., Gurr, S.J., Hirsch, P.R. and Mauchline, T.H. (2016). Expoitation of endophytes for sustainable agricultural intensification. Molecular Plant Pathology (in press).




Mehrabi, Z., McMillan, V.E., Clark, I. C., Hammond-Kosack, K.E., Preston, G., Hirsch, P.R. and Mauchline, T.H. (2016). Pseudomonas spp. diversity is negatively associated with suppression of the wheat take-all pathogen. Scientific Reports (in press).




Robinson, R.J., Fraaije, B.A., Clark, I.M., Jackson, R.W., Hirsch, P.R. and Mauchline, T.H. (2016). Wheat seed embryo excision enables the creation of axenic seedlings and Koch's postulates testing of putative bacterial endophytes. Scientific Reports (in press).



Hirsch P.R. and Mauchline T.H. (2015). The Importance Of The Microbial N Cycle In Soil For Crop Plant Nutrition. Advances In Applied Microbiology 93:45-71.



Mauchline, T.H., Chedom-Fotso, D., Chandra, G., Samuels, T., Greenaway, N., Backhaus, A.,McMillan, V., Canning, G., S. J. Powers, Hammond-Kosack, K.E., Hirsch, P.R., Clark, I.M., Mehrabi, Z., Roworth, J., Burnell, J., and J.G. Malone. (2015). An analysis of Pseudomonas genomic diversity in take-all infected wheat fields reveals the lasting impact of wheat cultivars on the soil microbiota. Environmental Microbiology 17(11):4764-78.



Robinson, R.J., Fraaije, B.A., Clark, I.M., Jackson, R.W., Hirsch, P.R. and Mauchline, T.H. (2015). Endophytic bacterial composition in wheat (Triticum aestivum) is determined by plant tissue type, developmental stage and soil nutrinet availability. Plant and Soil 05/2015; DOI:10.1007/s11104-015-2495-4.



Mauchline, T.H., Hayat, R., Roberts, R., Powers, S.J. and Hirsch P.R. (2014). Assessment of core and accessory genetic variation in Rhizobium leguminosarum symbiovar trifolii strains from diverse locations and host plants using PCR-based methods. Letters in Applied Microbiology. 59(2):238-46.



Hirsch, P.R., Mauchline, T.H. and Clark, I.M. (2013). Culture-independent molecular approaches to microbial ecology in soil and the rhizosphere. In: Molecular Microbial Ecology of the Rhizosphere Volume 1 & 2. Ed F.J. de Bruijn. John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118297674.ch5



Hirsch, P.R. and Mauchline, T.H. (2012). Who’s who in the plant root microbiome? Nature Biotechnology 30: 961-962.



Hirsch, P.R. and Mauchline, T.H. (2012). Plant-microorganism mutualism. In: Microbial Ecological Theory. Eds L.A. Ogilvie & P.R. Hirsch. Caister Academic Press, Norfolk, UK. p. 43-55.



Rao, U., Mauchline, T.H. and Davies K.G. (2012). The 16S rRNA gene of Pasteuria penetrans provides an early diagnostic of infection of root-knot nematodes (Meloidogyne spp). Nematology. Accepted for publication.



Mohan, S., Mauchline, T. H., Rowe, J., Hirsch, P.R., Davies, K.G. (2012). Pasteuria endospores from Heterodera cajani (Nematoda: Heteroderidae) exhibit inverted attachment and altered germination in cross-infection studies with Globodera pallida (Nematoda: Heteroderidae). FEMS Microbiol Ecol, Nov 12 doi: 10.1111/j.1574-6941.2011.01249.x. [Epub ahead of print].



Mauchline, T. H., Knox, R., Mohan, S., Powers, S. J., Kerry, B. R., Davies, K. G., Hirsch, P. R. (2011). Identification of new SNP- based markers for inter and intra-species discrimination of obligate bacterial parasites (Pasteuria spp.) of invertebrates.  Applied and Environmental Microbiology, 77, 6388-6394.



Schaff, J.E., Mauchline, T.H., Opperman, C. H., Davies, K.G. (2011). Exploiting Genomics to Understand the Interactions Between Root-Knot Nematodes and Pasteuria penetrans. In K.G. Davies and Y. Spiegal (ed), Biological Control of Plant-Parasitic Nematodes, Springer, Netherlands.



Mauchline, T.H., Mohan, S. Davies, K. G., Schaff, J. E., Opperman, C. H., Kerry, B. R., Hirsch, P. R. (2010). A method for release and multiple strand amplification of small quantities of DNA from endospores of the fastidious bacterium Pasteuria penetrans. Letters in Applied Microbiology, 50, 515-521.



Hirsch, P.R., Mauchline, T.H. and Clark, I.M. (2010). Culture-independent molecular techniques for soil molecular ecology. Soil Biology and Biochemistry,42, 878-887.



Karunakaran, R., Ramachandran, V. K., Seaman, J., East, A. K., Moushine , B. Mauchline, T. H., Prell, J., Skeffington, A., Poole, P. S. (2009). Transcriptomic analysis of the symbiotic interaction between Rhizobium leguminosarum b.v. viciae and host plants (Pisum sativa and Vicia cracca). Journal of Bacteriology. 191(12):4002-14



East, A.K., Mauchline, T. H. and Poole P.S. (2008). Biosensors for ligand detection. Adv Appl Microbiol. 64: 137-166.



Barr, M. East, A.K., Leonard, M., Mauchline, T.H. and Poole, P.S. (2008). In vivo expression technology (IVET) selection of genes of Rhizobium leguminosarum biovar viciae A34 expressed in the rhizosphere. FEMS Microbiol Lett. 282 (2): 219-227.



Mauchline, T. H., Fowler, J. E., East, A. K., Sartor, A. L., Cowie, A., Hosie, A. H. F., Poole, P. S., Finan, T. M. (2006). mapping the Sinorhizobium meliloti solute binding protein dependent transportome. PNAS, Nov 21;103 (47):17933-8. 



Young, J.P., Crossman, L.C., Johnston, A.W., Thomson, N.R., Ghazoui, Z.F., Hull, K.H., Wexler, M., Curson, A.R., Todd, J.D., Poole, P.S., Mauchline, T.H., East, A.K., Quail, M.A., Churcher, C., Arrowsmith, C., Cherevach, I., Chillingworth, T., Clarke, K., Cronin, A., Davis, P., Fraser, A., Hance, Z., Hauser, H., Jagels, K., Moule, S., Mungall, K., Norbertczak, H., Rabbinowitsch, E., Sanders, M., Simmonds, M., Whitehead, S., Parkhill, J. (2006).  The genome of Rhizobium leguminosarum has recognisable core and accessory components. Genome Biology, Apr 26; 7(4): R34.



Karunakaran, R., Mauchline, T.H., Hosie, A.H.F.,Poole P.S. (2005). A high throughput family of auto fluorescent proteins (AFP) and chromogenic reporter probe vectors for studying gene expression in Gram-negative bacteria in situ. Microbiology-UK, 151, 3249-56.



Mauchline, T. H., Kerry, B. R. & Hirsch, P. R. (2004). The biocontrol fungus Pochonia chlamydosporia shows nematode host preference at the infraspecific level. Mycological Research 108, 161-169.



Atkins, S. D., Mauchline, T. H., Kerry, B. R., Hirsch, P. R. (2004). Development of a transformation system for the nematophagous fungus Pochonia chlamydosporia. Mycological Research 108, 654-661.



Bourne, J. M., Karanja, P. K., Kalisz, H., Karanja, D. K., Mauchline, T. H., Kerry, B. R. (2004). Incidence and severity of damage caused by Meloidogyne spp. and isolation and screening of the nematophagous fungus Pochonia chlamydosporia from some of the main vegetable growing areas in Kenya. International Journal of Nematology 14, 111-122.



Kerry, B. R., Atkins, S. D., Mauchline, T. H., Morton, C. O., Hirsch, P. R. (2004). Variation in Pochonia chlamydosporia and its potential as a biological control agent for root-knot nematodes. IOBC/WPRS Bulletin 27(1), 127-132.



Atkins, S. D., Hidalgo-Diaz, L., Kalisz, H., Mauchline, T. H., Hirsch, P. R., Kerry, B. R. (2003). Development of a new management strategy for the control of root-knot nematodes (Meloidogyne spp) in organic vegetable production. Pest Management Science 59, 183-189.



Morton, C. O., Mauchline, T. H., Kerry, B. R., Hirsch, P. R. (2003). PCR-based DNA fingerprinting indicates host-related genetic variation in the nematophagous fungus Pochonia chlamydosporia. Mycological Research 107, 198-205



Mauchline, T. H., Kerry, B. R. & Hirsch, P. R. (2002). Quantification in soil and the rhizosphere of the nematophagous fungus Verticillium chlamydosporium by competitive PCR and comparison with selective plating. Applied and Environmental Microbiology 68, 1846-1853.



Kerry, B. R., Atkins, S. D., Mauchline, T. H., Hirsch, P. R. (2001). Measurement of the impact of Verticillium chlamydosporium on the dynamics of sedentary nematodes in the rhizosphere. IOBC/WPRS Bulletin 24(1), 73-76.



Hirsch, P. R., Atkins, S. D., Mauchline, T. H., Morton, C. O., Davies, K. G., Kerry, B. R. (2001). Methods for studying the nematophagous fungus Verticillium chlamydosporium in the root environment. Plant and Soil 232, 21-30.



Sosnowska, D., Mauchline, T. H., Bourne, J. M., Kerry, B. R. (2001). Screening of Polish, other European and tropical isolates of Verticillium chlamydosporium to assess their potential as biological control agents of root-knot nematodes. Journal of Plant Protection Research 41, 77-84.



Hirsch, P. R., Mauchline, T. H., Mendum, T. A., Kerry, B. R. (2000). Detection of the nematophagous fungus Verticillium chlamydosporium in nematode-infested plant roots using PCR. Mycological Research 104, 435-439.



























































PhD in Microbiology (University of Reading)


BSc in Applied Biology (University of Bath)

Measures of esteem


  • PhD co-supervisor: Rebekah Robinson - University of Reading
  • PhD co-supervisor: Rachel Parsonson - University of Reading


  • PhD co-supervisor: Glyn Barrett - University of Reading


  • Guest lecturer University of Reading - From 2007 to present: Medical Microbiology
  • Guest Lecturer University of Reading - From 2007 to present: Introductory Microbiology