Rothamsted Research

where knowledge grows

sparksc

Caroline Sparks

Role(s):
Transformation Manger
Plant Sciences
Harpenden
Phone:
+44 (0) 1582 763 133 ext: +44 1582 938831
Location:
Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ

Peer reviewed journals

  • S. Pearce, R. Savile, S. Vaughan, P. Chandler, E. Wilhelm, C. Sparks, N. Al-Kaff, A. Korolev, M. Boulton, A. Phillips, P. Hedden, P. Nicholson and S. Thomas (2011) Molecular characterisation of Rht-1 dwarfing genes in hexaploid wheat (Triticum aestivum). Submitted to Plant Physiology July 2011
  • J. Freeman, C. A. Sparks, J. West, P. R. Shewry and H. D. Jones (2011) Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotechnology Journal, 9: 788-796.
  • C. A. Sparks, J. Freeman and H. D. Jones (2011) Promoter validation from transgene expressino in wheat: a resource for vector design. Aspects of Applied Biology, 110: 28.
  • J. Freeman, C. A. Sparks, J. West, P. R. Shewry and H. D. Jones (2011) Temporal and spatial expression using a heat-shock promoter in transgenic wheat. Aspects of Applied Biology, 110: 85-91.
  • T. van Herpen, A. Riley, C. Sparks, H.D. Jones, C. Gritsch, E.H. Dekking, R.J. Hamer, D. Bosch, E.M.J. Salentijn, M.J.M. Smulders, P.R. Shewry and L. Gilissen (2008) Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development. Annals of Botany, 102: 331-342.
  • Gabriela M. Pastori, Alison Huttly, Jevon West, Caroline Sparks, Alejandro Pieters, Celina M. Luna, Huw D. Jones and Christine H. Foyer (2007) The maize Activator/Dissociation system is functional in Hexaploid wheat through successive generations. Functional Plant Biology, 34: 835–843.
  • H. Wu, C.A. Sparks & H.D. Jones (2006) Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium-mediated transformation. Molecular Breeding, 18: 195-208.
  • E. Mendoza de Gyves, C. Sparks, O. Sayanova, P Lazzeri, J.A. Napier and H.D. Jones (2004) Genetic manipulation of γ-linolenic acid (GLA) synthesis in commercial varieties of evening primrose (Oenothera Sp.). Plant Biotechnology Journal, 2: 351-357.
  • Huixia Wu, Caroline Sparks, Ben Amoah and Huw D. Jones (2003) Factors influencing successful Agrobacterium-mediated genetic transformation of wheat. Plant Cell Reports, 21: 659-668.
  • C.A. Sparks, C.K. Castleden, J. West, D.Z. Habash, P.J. Madgwick, M.J. Paul, G. Noctor, J. Harrison, R. Wu, J. Wilkinson, W.P. Quick, M.A.J. Parry, C.H. Foyer and B.J. Miflin (2001) Potential for manipulating carbon metabolism in wheat.  Annals of Applied Biology, 138: 33-45.
  • G. Pastori, M. Wilkinson, S. Steele, C. Sparks, H.D. Jones and M.A.J. Parry (2001) Age-dependent transformation frequency in elite wheat varieties. Journal of Experimental Botany, 52(357): 857-863.
  • B.K. Amoah, H. Wu, C. Sparks and H.D. Jones (2001) Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue. Journal of Experimental Botany, 52(358): 1135-1142.
  • E. Mendoza de Gyves, C.A. Sparks, A.F. Fieldsend, P.A. Lazzeri, and H.D. Jones (2001) High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron. Annals of Applied Biology, 138: 329-332.
  • G.Y. He, L. Rooke, M. Cannell, S. Rasco-Gaunt, C. Sparks, C. Lamacchia, F. Békés,  A.S. Tatham, P. Barcelo, P.R. Shewry, and P.A. Lazzeri  (1998) Current Status of Transformation in Bread and Durum Wheats and Modifications of Gluten Quality. Acta Agronimica Hungarica, 46(4).
  • A. Martinez, C. Sparks, C.A. Hart, J. Thompson and I. Jepson (1999) Ecdysone agonist inducible transcription in transgenic tobacco plants. The Plant Journal, 19(1): 97-106.
  • A. Martinez, C. Sparks, P. Drayton, J. Thompson, A. Greenland and I. Jepson (1999) Creation of ecdysone receptor chimeras in plants for controlled regulation of gene expression. Molecular and General Genetics, 261(3): 546-552.
  • K. Edwards, H. Thompson, D. Edwards, A. de Saizieu, C. Sparks, J. A. Thompson, A. J. Greenland, M. Eyers and W. Schuch (1992) Construction and characterisation of a yeast artificial chromosome library containing  three haploid maize genome equivalents. Plant Molecular Biology, 19: 299-308.
  • G. Nisbet, C. Sparks, C. Beech, P. Drayton, J. Thompson and R.J. Ellis (1990) Gene transfer and expression in cultured maize cells. Journal of Experimental Botany, 41: Supplement pP5-12.

 Book chapters

  • Caroline A. Sparks and Huw D. Jones (2008) “Biolistics Transformation of Wheat”. In: Transgenic Wheat, Barley and Oats; Production and Characterisation. Methods in Biotechnology Ed. Jones HD & Shewry PR. Humana Press Totowa, NJ. Chapter 4 p.71-92.
  • Huw D. Jones and Caroline Sparks (2008) “Selection of Transformed Plants”. In: Transgenic Wheat, Barley and Oats; Production and Characterisation. Methods in Biotechnology Ed. Jones HD & Shewry PR. Humana Press Totowa, NJ. Chapter 2 p.23-37.
  • Huw D. Jones and Caroline A. Sparks (2008) “Promoter sequences for defining transgene expression”. In: Transgenic Wheat, Barley and Oats; Production and Characterisation. Methods in Biotechnology Ed. Jones HD & Shewry PR. Humana Press Totowa, NJ. Chapter 11 p.171-184.
  • Huw D. Jones and Caroline A. Sparks (2008) “Stable Transformation of Plants”. In: Plant Genomics, Methods in Molecular Biology. Eds D Somers, P Langridge and JP Gustafson. Humana Press Totowa, NJ. Chapter 7 p.111-130.
  • Huw D. Jones, Angela Doherty and Caroline A. Sparks (2008) “Transient Transformation of Plants” In: Plant Genomics, Methods in Molecular Biology. Eds D Somers, P Langridge and JP Gustafson. Humana Press Totowa, NJ. Chapter 8 p.131-152.
  • Huw D. Jones, Caroline A. Sparks and Peter R. Shewry (2008) “Genetic Improvement of Wheat Quality”. Chapter 12 in Wheat Chemistry and Technology, AACC monograph.
  • C.A. Sparks and H.D. Jones (2004) “Transformation of wheat by biolistics” In: Transgenic crops of the world. Ed. Ian S. Curtis. Kluwer Academic publishers. Chapter 2 p.19-33.

 

Qualifications

1987:

BSc in Biological Sciences (University of York)