New resource will greatly accelerate scientific discovery and the rate of wheat improvement

  • 16
  • JAN
  • 2017

A team of scientists in the UK and USA have generated a new groundbreaking resource of ten million mutations in bread and pasta wheat varieties.

Researchers and breeders can search the public wheat database online to identify changes in their genes of interest and request seeds to improve the nutrition and production of wheat worldwide. They anticipate this will speed up the development of the wheat crop with highly sought-after traits, including disease resistance and increased yield.

Since the dawn of agriculture, wheat has been a major dietary source of calories and protein for humans. Breeding of better bread and pasta wheat varieties is key for sustaining global food security. The modern-day cultivated wheat species harbor polyploid genomes, meaning that each plant has multiple functional copies of each gene.1 Therefore, genetic variation (such as mutations) in one gene copy are frequently masked, or hidden, by functional copies within the other genomes.

Scientists from the Earlham Institute (EI), the John Innes Centre (JI), Rothamsted Research (RRes, UK) and the University of California, Davis (UC Davis, USA), funded by BBSRC, USDA and HHMI, have catalogued ten million mutations by sequencing 400 billion bases of DNA from 2735 mutant wheat lines. These hidden mutations are likely to disrupt more than 90% of the pasta and bread wheat genes.

Wheat researchers from all over the world JI are using this publicly available collection of mutants with close to 3,000 individual seed stocks already distributed. The first results are starting to surface. Using these mutations, improved nutritional wheat varieties with larger grains and more dietary value have been developed. Scientists are also utilising this collection to characterise gene functions that control wheat flowering and adaptation to new and changing environments.

First author Dr Ksenia Krasileva, Group Leader at EI and The Sainsbury Laboratory, said: “Breeding better wheat is an ongoing major challenge that we need to meet to keep agriculture sustainable and produce varieties that are well adapted to changes in global climate. Within the polyploid wheat genomes, there is a highly-significant amount of genetic variation that researchers can unearth that is commonly hidden - masked by multiple copies of each gene in the different wheat genomes. We need to identify and combine variation from different wheat genes in order to reveal the crop’s most beneficial changes.

“Our study produced a well-catalogued collection of over 10 million changes in wheat genes that breeders and researchers can use today to improve wheat. Essentially, this work will help us to better understand the functions of wheat mutations that have taken place in our wheat varieties, which should hopefully aid worldwide production of wheat.”

Dr Cristobal Uauy, Crop Genetics Project Leader at JIC, added: “This study provides immediate access to a treasure trove of genetic variation to breeders and researchers. Critically, this variation and the novel combinations that will be generated have not previously been utilised in traditional wheat breeding programmes. This represents an unprecedented opportunity to make significant progress in wheat improvement to address a series of challenges facing sustainable crop production.”

Dr Andy Phillips, Rothamsted Research, said: “Since before the Green Revolution, mutation breeding has been very valuable in less complex crops such as rice, which as a diploid does not have additional copies of most genes. Our work provides a valuable non-GM resource that will allow scientists and breeders to identify and combine mutations in the multiple copies of wheat target genes, allowing the effects on the plant to be observed and exploited to accelerate crop improvement.”

The scientific paper “Uncovering hidden variation in polyploid wheat” is published in Proceedings of the National Academy of Sciences (PNAS).

1. The breeding of better varieties of bread and pasta wheat is key for sustaining global food security. Pasta wheat has two pairs of seven chromosomes (four genomes - tetraploid), while bread wheat has three such pairs (six genomes - hexaploid). 

About Earlham Institute

The Earlham Institute (EI) is a world-leading research institute focusing on the development of genomics and computational biology. EI is based within the Norwich Research Park and is one of eight institutes that receive strategic funding from Biotechnology and Biological Science Research Council (BBSRC) - £6.45M in 2015/2016 - as well as support from other research funders. EI operates a National Capability to promote the application of genomics and bioinformatics to advance bioscience research and innovation.

EI offers a state of the art DNA sequencing facility, unique by its operation of multiple complementary technologies for data generation. The Institute is a UK hub for innovative bioinformatics through research, analysis and interpretation of multiple, complex data sets. It hosts one of the largest computing hardware facilities dedicated to life science research in Europe. It is also actively involved in developing novel platforms to provide access to computational tools and processing capacity for multiple academic and industrial users and promoting applications of computational Bioscience. Additionally, the Institute offers a training programme through courses and workshops, and an outreach programme targeting key stakeholders, and wider public audiences through dialogue and science communication activities.

About the John Innes Centre

The John Innes Centre is an independent, international centre of excellence in plant science and microbiology.

Our mission is to generate knowledge of plants and microbes through innovative research, to train scientists for the future, to apply our knowledge of nature’s diversity to benefit agriculture, the environment, human health and wellbeing, and engage with policy makers and the public.

To achieve these goals we establish pioneering long-term research objectives in plant and microbial science, with a focus on genetics. These objectives include promoting the translation of research through partnerships to develop improved crops and to make new products from microbes and plants for human health and other applications. We also create new approaches, technologies and resources that enable research advances and help industry to make new products. The knowledge, resources and trained researchers we generate help global societies address important challenges including providing sufficient and affordable food, making new products for human health and industrial applications, and developing sustainable bio-based manufacturing.

About The Sainsbury Laboratory, Norwich

The Sainsbury Laboratory (TSL) is a world-leading research centre focusing on making fundamental discoveries about plants and how they interact with microbes. TSL not only provides fundamental biological insights into plant-pathogen interactions, but is also delivering novel, genomics-based, solutions which will significantly reduce losses from major diseases of food crops, especially in developing countries. TSL is an independent charitable company and receives strategic funding from the Gatsby Charitable Foundation with the balance coming from competitive grants and contracts from a range of public and private bodies, including the European Union (EU), Biotechnology and Biological Sciences Research Council (BBSRC) and commercial and charitable organisations

About Rothamsted Research
Rothamsted Research is the longest-running agricultural research institute in the world. We work from gene to field with a proud history of ground-breaking discoveries, from crop treatment to crop protection, from statistical interpretation to soils management. Our founders, in 1843, were the pioneers of modern agriculture, and we are known for our imaginative science and our collaborative influence on fresh thinking and farming practices.
Through independent science and innovation, we make significant contributions to improving agri-food systems in the UK and internationally. In terms of the institute’s economic contribution, the cumulative impact of our work in the UK was calculated to exceed £3000 million a year in 20151. Our strength lies in our systems approach, which combines science and strategic research, interdisciplinary teams and partnerships.
Rothamsted is also home to three unique resources. These National Capabilities are open to researchers from all over the world: The Long-Term Experiments, Rothamsted Insect Survey and the North Wyke Farm Platform.
We are strategically funded by the Biotechnology and Biological Sciences Research Council (BBSRC), with additional support from other national and international funding streams, and from industry. We are also supported by the Lawes Agricultural Trust (LAT).
For more information, visit; Twitter @Rothamsted
1Rothamsted Research and the Value of Excellence: A synthesis of the available evidence, by Séan Rickard (Oct 2015)

The Biotechnology and Biological Sciences Research Council is part of UK Research and Innovation, a non-departmental public body funded by a grant-in-aid from the UK government.
BBSRC invests in world-class bioscience research and training on behalf of the UK public. Our aim is to further scientific knowledge, to promote economic growth, wealth and job creation and to improve quality of life in the UK and beyond.
Funded by government, BBSRC invested £469 million in world-class bioscience in 2016-17. We support research and training in universities and strategically funded institutes. BBSRC research and the people we fund are helping society to meet major challenges, including food security, green energy and healthier, longer lives. Our investments underpin important UK economic sectors, such as farming, food, industrial biotechnology and pharmaceuticals.
More information about BBSRC, our science and our impact.
More information about BBSRC strategically funded institutes

About LAT
The Lawes Agricultural Trust, established in 1889 by Sir John Bennet Lawes, supports Rothamsted Research’s national and international agricultural science through the provision of land, facilities and funding. LAT, a charitable trust, owns the estates at Harpenden and Broom's Barn, including many of the buildings used by Rothamsted Research. LAT provides an annual research grant to the Director, accommodation for nearly 200 people, and support for fellowships for young scientists from developing countries. LAT also makes capital grants to help modernise facilities at Rothamsted, or invests in new buildings.